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. 2022 Jun 27;20:288. doi: 10.1186/s12967-022-03456-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — METTL3 is a target gene of miR-493-5p. A, GO functional enrichment analysis of the candidate genes. B, Differential expression of METTL3 in AML samples in TCGA and GTEx. The red box plot represents disease samples, and the gray box plot represents normal samples. C, starBase was used to predict the binding site of miR-493-5p to METTL3. D, Dual luciferase reporter assay was used to detect the binding of miR-493-5p to METTL3. E, METTL3 expression in bone marrow samples of AML patients (n = 35) and healthy individuals (n = 35) determined by RT-qPCR. F, Correlation analysis between miR-493-5p expression and METTL3 expression in the bone marrow samples of AML patients (n = 35). G, METTL3 expression in HS-5, HL-60 and Molm13 cells and HL-60 R and Molm13 R cells measured by RT-qPCR. H, METTL3 expression in HL-60 and Molm13 cells and HL-60 R and Molm13 R cells upon miR-493-5p inhibitor/mimic determined by RT-qPCR. * p < 0.05. Cell experiments were performed three times independently