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. 2022 Jun 27;240:114570. doi: 10.1016/j.ejmech.2022.114570

Fig. 2.

Fig. 2

Inhibition curves of MPI11-28 on MPro. Triplicate experiments were performed for each compound. For all experiments, 20 or 10 nM MPro was incubated with an inhibitor for 30 min before 10 μM Sub3 was added. The MPro-catalyzed Sub3 hydrolysis rate was determined by measuring linear increase of product fluorescence (Ex: 336 nm/Em: 455 nm) for 5 min.