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. Author manuscript; available in PMC: 2022 Sep 23.
Published in final edited form as: J Med Chem. 2021 Sep 1;64(18):13510–13523. doi: 10.1021/acs.jmedchem.1c00726

Figure 2.

Figure 2.

Radioligand competition binding at human 5-HT1ARs and 5-HT2BRs. Ordinates: percent specific binding of the radioligand to receptors expressed in cell membranes (described in the Experimental Section). Abscissae: concentrations of test ligands (log scale). The left panel shows percent specific binding of [3H]8-OH-DPAT at 5-HT1ARs. The right panel shows percent specific binding of [125I]DOI at 5-HT2BRs. These experiments were conducted in duplicate and repeated twice (N = 2), and the data shown were obtained by Eurofins. We conducted additional affinity tests to validate these results, and the binding curves are shown in the Supporting Information (Figure S21).