Figure 20:

Experimental and simulated permeabilization of LUVs. We simulate vesicle leakage using simple numerical models. A: Transient leakage has a constant exponential rise toward a final value that itself depends on the P:L in the experiment. Many leakage experiments have behavior like this. The exponential rise assumes that the fraction of remaining entrapped contents released per unit time is constant. The plot on the right shows potency profile in a semi-log plot. B: Simple equilibrium leakage model assumes an exponential rise towards 100% release where the rate (the fraction of remaining entrapped contents released per unit time) is a function of peptide concentration. This behavior is rarely observed. The plots on the right shows potency profile at 30 minutes in a semi-log plot. C: Hybrid leakage, assumes that there is a major component of transient leakage, followed by a low level steady equilibrium release. Many peptides have this behavior. D: Real experimental leakage curves for various MPPs taken from the authors’ manuscripts. Real curves like all three of these simulations are seen in this set. The plots on the right shows various potent curves taken from the authors’ manuscripts. They range from high potency MPPs (e.g. alamethicin) that release ~100% of contents at P:L=1:2000, to some AMPs or analogs that release almost nothing at P:L of 1:10. Peptides, lipid compositions and other details vary in these curves.