Table 1. Detection of CTCs in melanoma patients with PAFC in vivo.

For P1 to P15: CTCs per milliliter in vivo versus in vitro, Spearman correlation = +0.570 (P = 0.0266); CTC patient result in vivo versus in vitro, concordance = 3 of 15 (20%) and Cohen’s κ ± SE = 0.02 ± 0.13. For P19 to P28: CTCs per milliliter in vivo versus in vitro, Spearman correlation = +0.527 (P = 0.1172); CTC patient result in vivo versus in vitro, concordance = 2 of 10 (20%) and Cohen’s κ ± SE = 0.00 ± 0.16.

CTCs	In vivo PAFC
Subject no.	Blood volume*	Number detected	CTCs per milliliter†	Patient result‡
P1	234	276	1.178 ± 0.071	Yes
P2	117	238	2.033 ± 0.132	Yes
P3	842	120	0.143 ± 0.013	Yes
P4	690	32	0.046 ± 0.008	Yes
P5	150	0	0.000 ± 0.000	No
P6	501	3	0.006 ± 0.003	Yes
P7	667	15	0.022 ± 0.006	Yes
P8	954	72	0.075 ± 0.009	Yes
P9	658	43	0.065 ± 0.010	Yes
P10	509	51	0.100 ± 0.014	Yes
P11	164	5	0.030 ± 0.014	Yes
P12	200	77	0.385 ± 0.044	Yes
P13	660	23	0.035 ± 0.007	Yes
P14	516	20	0.039 ± 0.009	Yes
P15	221	3	0.014 ± 0.008	Yes
P16	230	462	2.009 ± 0.093	Yes
P17	682	298	0.437 ± 0.025	Yes
P18	235	282	1.200 ± 0.071	Yes
P19	242	273	1.129 ± 0.068	Yes
P20	91	208	2.298 ± 0.159	Yes
P21	197	11	0.056 ± 0.017	Yes
P22	295	171	0.580 ± 0.044	Yes
P23	174	219	1.257 ± 0.085	Yes
P24	301	63	0.210 ± 0.026	Yes
P25	33	49	1.472 ± 0.210	Yes
P26	163	52	0.319 ± 0.044	Yes
P27	266	15	0.056 ± 0.015	Yes
P28	80	22	0.274 ± 0.058	Yes

^*Blood volume is in milliliters.

^†Point estimate ± SE of the estimate.

^‡Patient result was calculated as “yes” if the patient’s CTC rate (in CTCs per milliliter) was elevated above the false-positive CTC rate by >2 SEs of the difference. False-positive CTC rates ± SEs were 0.0040 ± 0.0008 CTCs/ml in vivo and 0.465 ± 0.147 CTCs/ml in vitro.