Figure 5.

Response of stored ERYs to β-cell-like secretions from rat INS-1 cells as determined by ERY-derived ATP. AS-1N-PSSN samples (green bars) responded to β-cells in a manner similar to the direct addition of exogenous C-peptide and Zn²⁺. ERYs stored in the AS-1-PSSH solutions (gray bars) responded with significantly less release of ATP, suggesting less binding of the C-peptide and Zn²⁺ to these ERYs in hyperglycemic solutions. Response of AS-1-PSSN samples (red bars) to secretions followed a similar pattern as data shown in other figures; specifically, the high glucose levels in the AS-1 were overcome upon addition to PSSN. However, beyond day 8 of storage in the AS-1, the ERYs were unable to fully recover in the normoglycemic PSSN. Error bars are ±SEM, n=3 for all, *p<0.05 to AS-1N-PSSN day 1.