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Effect of DNA quality on the amplification of SSU rDNA by PCR. Standard curves show plots of CT versus log number of gene copies for SSU rDNA from H. volcanii, as estimated by 5′-nuclease assays using the ARCH1 set. Open circles, SSU rDNA in supercoiled plasmids; closed circles, genomic DNA; open squares: SSU rDNA in linearized plasmids.
