FIG. 3.

Estimation of the percentage of a Synechococcus sp. in mixtures with genomic DNA of strain R2A57. The x axis shows expected values calculated using various mixture proportions and the numbers of SSU rDNA copies of Synechococcus sp. strain WH7803 and strain R2A57 per microliter prior to mixing. The y axis shows the percentage of SSU rDNA from Synechococcus sp. strain WH7803 estimated by 5′-nuclease assays using set PHPICO. Total SSU rDNA was estimated by 5′-nuclease assays using set BACT2. Error bars indicate 1 standard deviation from the mean of triplicate reactions. The line represents a theoretical line assuming a 100% match in the values.