FIG. 1.

One-pot multienzyme (OPME) GlcNAc (A), Gal (B), and Neu5Ac (C) activation and transfer systems.^{269, 270} Enzyme abbreviations: BiNahK, B. infantis N-acetylhexosamine-1-kinase;³¹⁷ PmGlmU, P. multocida N-acetylglucosamine 1-phosphate uridylyltransferase;³¹⁸ PmPpA, P. multocida inorganic pyrophosphatase;³¹⁸ GlcNAcT, N-acetylglucosaminyltransferase; NmLgtA, Neisseria meningitidis β1–3-N-acetylglucosaminyltransferase;³¹⁹ EcGalK, E. coli K-12 galactose kinase;³²⁰ BLUSP: B. longum UDP-sugar synthase;³²⁰ GalT, galactosyltransferase; NmLgtB, Neisseria meningitidis β1–4-galactosyltransferase;³²¹ NmCSS, Neisseria meningitidis CMP-sialic acid synthetase;²⁹³ SiaT, sialyltransferases; PmST1 M144D, Pasteurella multocida α2–3-sialyltransferase 1 M144D mutant;²⁹⁶ Pd2,6ST, Photobacterium damselae α2–6-sialyltransferase;^{309, 322} CstII, Campylobacter jejuni α2–8-sialyltransferase II.^{323, 324}