Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 2;82(11):2113–2131.e8. doi: 10.1016/j.molcel.2022.04.027

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Properties of the CENP-LN vault

(A) The isolated CENP-LN vault with highlighted CENP-N pyrin domain (∼80 residues, greencyan), CENP-N LNHD (LN homology domain), dimerization domains of CENP-N and CENP-L, and CENP-L LNHD. A 2-fold pseudosymmetry axis (interrupted line with red oval) relates the LNHDs.

(B) Top: Rotated cartoon model of the CENP-N-terminal region bound to a CENP-A nucleosome (PDB: 6C0W) in two orthogonal orientations (Pentakota et al., 2017). The pyrin domain binds the L1 loop of CENP-A (light blue) and the LNHD to DNA. H2A C-terminal tails were removed for clarity. Bottom: Superposition of CENP-N in 6C0W to CENP-N in human CCAN predicts a steric clash of the second DNA gyre (boxed) onto pillar 2. The vault can only accept one gyre of DNA.

(C) Cartoon model of the RdgC homodimer highlighting the LNHDs and other structural elements.

(D) The LNHDs of CENP-LN displayed in absence of other domains (dimerization, pyrin) and modeled with 15-bp DNA from the 6C0W structure (B) positioned identically relative to CENP-N. The 2-fold pseudosymmetry axis between LNHDs coincides with the 2-fold pseudosymmetry axis of DNA.

(E) A 90°-rotated view of the same object.