Figure 6.

Comparison of CCAN disassembly rates

(A) Degradation of CENP-C endogenously tagged with a EYFP-AID cassette (Fachinetti et al., 2015) was induced by addition of indole acetic acid (IAA, auxin). A complete time course is shown in Figure S15. Shown here is the 8-h time point. Levels of CENP-A, CENP-HK, and CREST were also monitored. Scale bar, 10 μm.

(B) As in (A), but with levels of CENP-T monitored instead of CENP-HK. Scale bar, 10 μm.

(C) Quantification of the experiments in (A) and (B). Residual levels (expressed as fractions of control), number of kinetochores, and number of cells (in parentheses) are indicated. Levels of CENP-A and CENP-C^YFP-AID from both experiments were quantified. Levels of CENP-T and CENP-HK were quantified from the individual experiments in (A) and (B). Red bars represent average fluorescence intensities and SD of quantified centromere foci from two technical repeats.

(D) Degradation of CENP-A endogenously tagged with a EYFP-AID cassette and of CENP-C endogenously tagged with an RFP-AID cassette (Hoffmann et al., 2020) at the 8-h time point after addition of IAA (see Figure S15). Levels of CENP-HK and CREST were also monitored. Scale bar, 10 μm.

E) As in (D), but with levels of CENP-T monitored. Scale bar, 10 μm.

F) Quantification of the experiments in (D) and (E) was performed and displayed precisely as described for (C).