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. 2022 Jun 23;15(6):dmm049538. doi: 10.1242/dmm.049538

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Spatial analysis revealed the aggressive proliferation of CSC-like populations in the lymph node metastasis. (A) Spatial transcriptomics analysis of human (cancer cell) clusters in the primary tumor and the lymph node metastasis. (B) Cell cycle phase of cancer cells in the primary tumor and lymph node metastases. (C) Spatial transcriptomics analysis of mouse (stromal cell) clusters in the primary tumor and lymph node metastasis. (D) Spatial analysis of human (cancer cell) clusters with the chi-squared test; *P<0.05; n.s., not significant. (E) Sunburst plot of the cell cycle in human cancer cell clusters. (F) Bar plot of cell cycle phases in the primary tumor and lymph node metastasis. Fisher's exact test; *P<0.05; **P<0.01; n.s., not significant.