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. 2022 Jun 23;11(1):356–365. doi: 10.1080/21623945.2022.2085417

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Immunohistochemistry analysis of adipogenic differentiation after 21 days in culture. Immunohistochemical analysis of the expression of Cyt c (AF594-labelled, red) and mTor (AF488-labelled, green) was performed in ADSCs cultured in the presence of adipogenic differentiation medium (DM), or in DM in the presence of vitamin D (DM+VIT), or in DM in the presence of metformin (DM+MET), or in DM with both metformin and vitamin D (DM+VIT+MET), as compared to control untreated cells (CTRL). The figures are representative of different independent experiments. For each differentiation marker, fields with the highest yield of positively stained cells are shown. Relative intensity was measured with ImageJ software. Nuclei are labelled with 4,6-diamidino-2-phenylindole (DAPI, blue). Scale bars: 40 µm.