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. 2022 Jun 23;11(1):366–378. doi: 10.1080/21623945.2022.2089394

Figure 4.

Figure 4.

Examination of the effects of cixutumumab (A12), a specific antibody leading to internalization and degradation of the insulin like growth factor-1 receptor (IGF-1 R) on expression of mRNAs encoding genes important in fatty acid and cholesterol metabolism. Male C57BL/6 J mice were fed a high fat diet for 6-weeks and received 10 mg/Kg A12 or isotype control (IC) every 3 days by intraperitoneal injection for 3 weeks, 21 days after commencing diet: (a) No difference in expression of pparg in epididymal white adipose tissue of cixutumumab-treated mice compared to isotype control-treated mice (n = 9–12). (b) No difference in mRNAs of Mttp, Hmgr, Fas, Srebp1c or Acc1 in brown adipose tissue of cixutumumab-treated mice compared to isotype control-treated mice. (c) Reduced mRNAs of Mttp, Hmgr and Acc1 in subcutaneous white adipose tissue of cixutumumab-treated mice compared to isotype control-treated mice (n = 7–10). (d) Reduced mRNA of Mttp in epididymal WAT of cixutumumab-treated mice compared to isotype control-treated mice (n = 5–7). Data expressed as mean (SEM),* denotes P < 0.05; **P < 0.005; #P < 0.05. n denotes number of mice per group, comparisons made using unpaired student's t test.