Table 2.
qPCR primers used in the study.
| Gene | Forward primer (5’ to 3’) | Reverse primer (5’ to 3’) |
|---|---|---|
| 18s | GATGCTCTTAGCTGAGTGT | GCTCTGGTCCGTCTTG |
| Ucp1 | CTTTGCCTCACTCAGGATTGG | ACTGCCACACCTCCAGTCATT |
| AdipoQ | GTATCGCTCAGCGTTC | CGTTGACGTTATCTGCAT |
| Lep | CATTTCACACACGCAGT | GGAGGTCTCGGAGATTC |
| Tnfa | CCACCACGCTCTTCTGTCTAC | AGGGTCTGGGCCATAGAACT |
| Prdm16 | TGACGGATACAGAGGTGTCAT | ACGCTACACGGATGTACTTGA |
| Cidea | AATAGCCAGAGTCACCTTCG | GGATGGCTGCTCTTCTGTAT |
| Pparg | CACAATGCCATCAGGTTTGG | GCTGGTCGATATCACTGGAGATC |
| rps29 m | GTCTGATCCGCAAATACGGG | AGCCTATGTCCTTCGCGTACT |
| Mttp | ATACAAGCTCACGTACTCCACT | TCCACAGTAACACAACGTCCA |
| Hmgr | AGCTTGCCCGAATTGTATGTG | TCTGTTGTGAACCATGTGACTTC |
| Srebpc | GATGTGCGAACTGGACACAG | CATAGGGGGCGTCAAACAG |
| Adipoq | GCAGAGATGGCACTCCTGGA | CCCTTCAGCTCCTGTCATTCC |
| Ppara | TATTCGGCTGAAGCTGGTGTAC | CTGGCATTTGTTCCGGTTCT |
| Cpt1a | CTCAGTGGGAGCGACTCTTCA | GGCCTCTGTGGTACACGACAA |
| Acc1 | GATGAACCATCTCCGTTGGC | GACCCAATTATGAATCGGGAGTG |
| Fas | TCCTGGGAGGAATGTAAACAGC | CACAAATTCATTCACTGCAGCC |
| Chrebp | TGCTTGAGCCTGGCTTACAGTG | AGGCCTTTGAAGTTCTTCCACTTG |
| Cyp7a1 | CAGGGAGATGCTCTGTGTTCA | AGGCATACATCCCTTCCGTGA |
| Hif1a | ACCTTCATCGGAAACTCCAAAG | CTGTTAGGCTGGGAAAAGTTAGG |