Improving Estimates of Compensatory cis–trans Regulatory Divergence

In this article, I regretfully neglected to cite a paper [1] in which the authors proposed separating RNA-seq technical replicates (the same library sequenced on different lanes) to estimate cisand transcomponents of gene expression divergence. While similar in spirit to the approach that I proposed, it should be noted that separating technical but not biological replicates will still lead to an artifactual negative cis–transcorrelation if there is any error in allele-specific expression estimates driven either by biological variation among samples or by variation in library construction (e.g., due to PCR ‘jackpots’).