FIGURE 4:

Synthetically clustered Num1 EFLM mutants rescue defects in dynein function observed in the absence of mitochondrial inheritance. (A, B) Representative fluorescence microscopy images of mito^AID cells expressing Num1-yEGFP, Num1ΔPH-yEGFP, PAN, PAN-CC, PANΔEF, and PAN^D315A and mito-TagBFP (magenta) grown in the absence (A) and presence (B) of 1 mM auxin. Whole-cell max projections are shown. The cell cortex is outlined with a dashed white line. Bar, 2 µm. (C) Quantification of misoriented spindles for mito^AID cells expressing Num1, PAN, PANΔEF, or PAN^D315A (as described in Figure 2A) in the absence and presence of auxin, as indicated under the graph. Large buds with spindles >1.25 μm were scored. n ≥ 292 spindles from three independent experiments of ≥78 spindles per replicate. Quantification of the percent of cells with misoriented spindles is shown as the mean ± SD with each dot representing an individual replicate. p value comparisons are designated by brackets. *** p < 0.0001, ** p < 0.001; n.s., not significant.