FIGURE 1:

Paxillin KO breast tumor mouse develops fewer lung metastases. (A) Paxillin expression does not significantly affect total tumor size; n = number of animals. (B) Western blot showing paxillin is absent in KO tumor and isolated tumor cell lysates. (C) Paxillin does not affect tumor latency; n (animals) (paxillin WT) = 39, n (paxillin KO) = 36. (D) Paxillin is depleted only in KO tumor cells and not stroma cells as visualized by immunofluorescence staining of tumor sections. T, tumor; S, stroma. (E) Whole lungs. White arrows show surface metastases. (F) H&E staining of lung sections. Lung metastases are highlighted with dashed circles. (G) Quantification of total number of lung metastases; n = 6 animals per genotype. A Student’s t test was performed for statistical analysis. Data represent mean ± SEM *< 0.05.