FIGURE 3:

Paxillin KO tumor organoids are less invasive. (A) Illustration of the Fiji-generated masks of tumor organoids. A high degree of circularity of the organoid correlates with low invasiveness. (B) Representative confocal images of tumor organoids. Arrows point to collective cell protrusions in paxillin WT organoids; arrowheads point to individual tumor cells in paxillin KO organoids. The asterisk indicates a hyper mesenchymal cell in a paxillin KO organoid. (C) Quantification of organoid circularity. Three animals per genotype; n (paxillin WT) = 172 organoids, n (paxillin KO) = 215 organoids. (D) Quantification of the average number of collective protrusions per organoid; n = 3 animals per genotype. (E) Quantification of the average number of single cells per field; n = 5 animals per genotype. A Student’s t test was performed for statistical analysis for C, D. A Mann–Whitney U test was performed for E. Data represent mean ± SEM *< 0.05, **< 0.01, ***< 0.001.