FIGURE 6:

MT acetylation is important for AJ organization in tumor cell islands and for tumor organoid invasion. (A) Representative images of paxillin WT and paxillin KO tumor cells in 2D treated with tubacin (2 μM) and stained for E-cadherin, vinculin, and F-actin. (B) Quantification of the percentage of mature AJs and disorganized AJs; n = 3 animals per genotype. (C) Representative confocal images of 3D tumor organoids treated with DMSO or tubacin (2 μM). Both paxillin WT and paxillin KO organoids showed high levels of acetylated-tubulin after tubacin treatment. Tubacin-treated paxillin KO organoids showed increased collective cell invasion as compared with the DMSO-treated paxillin KO organoids. Arrows point to collective cell invasion. (D) Quantification of organoid circularity; n = 3 animals per genotype. Each dot represents one organoid. A one-way ANOVA analysis with Tukey’s multiple comparison was performed for statistical analysis. Data represent mean ± SEM *< 0.05, **< 0.01, ***< 0.001.