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. Author manuscript; available in PMC: 2022 Jun 27.
Published in final edited form as: Exp Eye Res. 2018 Jun 26;176:29–39. doi: 10.1016/j.exer.2018.06.026

Fig. 3. Disruption of polarized RPE cells in eyes injected with AAV-CBA-Cas9 and AAV-Nhe8-KO sgRNAs.

Fig. 3.

Retinal frozen sections of AAV-injected eyeballs were characterized by co-staining with DAPI (blue), phalloidin (red) and anti-Ezrin (green). Left images are controls from AAV-CBA-Cas9 and AAV-control-sgRNA injected eye in low magnification (A) and high magnification (B), which revealed uniform apical distribution of ezrin staining signals in normal polarized RPE. Two middle images of (A) and (B) are from the other eye of the same mouse injected with AAV-CBA-Cas9, AAV-Nhe8-KO sgRNAs-P1/P2 and AAV-Nhe8-KO sgRNAs-E2/E5/E6. The right images of (A) and (B) are from another mouse eye injected with AAV-CBA-Cas9, AAV-Nhe8-KO sgRNAs-P1/P2 and AAV-Nhe8-KO sgRNAs-E2/E5/E6. In addition to a loss of outer nuclear layer, the middle and right images showed disrupted or mislocalized ezrin signals in RPE cells (white arrowheads), as well as aberrant cells (white arrows) in the subretinal space. RPE: retinal pigment epithelium; ONL: outer nuclear layer; INL: inner nuclear layer; GC: ganglion cell layer. Scale bars for all images: 50 μm.