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. Author manuscript; available in PMC: 2022 Jun 27.
Published in final edited form as: Exp Eye Res. 2018 Jun 26;176:29–39. doi: 10.1016/j.exer.2018.06.026

Fig. 5. Primary RPE cells isolated from wild-type albino B6-white mice. RPE cells were infected with AAV5-CBA-NHE8 and stained with NHE8 and intracellular organelle markers.

Fig. 5.

(A) Co-staining of anti-NHE8 (red) and anti-TGN46 (green) revealed co-localization of NHE8 and the Trans-golgi marker. Due to the weak endogenous NHE8 staining, we used the AAV-NHE8 to infect the cells to achieve more robust NHE8 staining. In the two cells (white arrows) with the most intense NHE8 staining, the red NHE8 signals were prominently expressed near nuclear, while the signals were also expressed throughout the cytosol. Co-staining with the Trans-golgi marker TGN46 revealed co-localized peri-nuclear NHE8 expression with the Golgi marker. (B) Co-staining of anti-NHE8 (red) and anti-calreticulin that labels endoplasmic reticulum revealed the prominent peri-nuclear NHE8 signals were not co-localized with ER, although the cytosolic NHE8 might show some co-localization with the ER marker. Scale bar: 20 μm.