Table 1. Table of Chlamydomonas Histone Proteoforms Identified by TDMS in This Studya.
| H1 | H1.1Nα-ac | acH1.2Nα-ac | |||
| H2A | H2A.0Nα-ac | H2A.1 | H2A.1Nα-ac | H2A.1Nα-acK5ac | H2A.1Nα-acK188ac |
| H2A. 2 | H2A.2Nα-ac | H2A.2Nα-acK5ac | H2A.2Nα-acK188ac | H2A.3 | |
| H2A.3Nα-ac | H2A.3Nα-acK5ac | H2A.4Nα-ac | H2A.ZNα-ac | H2A.ZNα-acK6acK14ac | |
| H2A.ZNα-acΔ143 | H2A.ZNα-acΔ142-143 | ||||
| H2B | H2BNα-me3.1c | H2BNα-me3.2c | H2BNα-me3.3c | H2BNα-me3.5c | H2BNα-me3.8c |
| H2BNα-me3.9c | H2BNα-me3.11c | H2BNα-me3.12c | H2BNα-me3.13c | H2BNα-me3.14c | |
| H2BNα-me3.15c | H2BNα-me3.v1 | H2BNα-me3.K7ac | H2BNα-me3.v1K11ac | H2BNα-me3.v1K7acK11ac | |
| H2BNα-me3.v1K7ac K11acK12ac | H2BNα-me3.v1K7acK11acK12acK16ac | ||||
| H3 | H3 | H3K4b/9me1 | H3K4me1 + 14 Da (K35-Q86) | H3K4/9me1+ 28 Da (K27-M119) | H3K4me1 + 42 Da (T28-Y40) |
| H3K4me1 + 56 Da (P37–F57) | H3K4me1 + 71 Da (L20-I118) | H3K4me1 + 84 Da (T28-T117) | H3K4me1 + 98 Da (K23-L99) | H3K4me1 + 112 Da (A23-R115) | |
| H3K4me1 + 125 Da (Q54-F77) | H3K4me1 + 140 Da (K23-T44) | H3K4me1 + 155 Da (K23-D105) | H3K4me1 + 167 Da (A25-F83) | H3K4me1 + K18ac +140 Da (Q19-L91) | |
| H3K9me1 + 197 Da (K23–I118) | H3K4/9me1 + 211 Da (G32-F103) | H3K4me3K9acK14acK18ac +70 Da (Q19-I118) | H3K4me3K9acK14ac +128 Da (Q23 - T79) | H3K4/9me1 + 255 Da unlocalized | |
| H3K4me3K9acK14acK18ac +115 Da (Q19-F77) | H3K4me3 + 174 Da (Q19-I118) or H3K4me1 + 283 Da (Q19-I118) | ||||
| H4 | H4Nα-ac | H4Nα-acK79me1 | H4Nα-acR3me1K79me1 | H4Nα-acK5acK79me1 | H4Nα-acK8acK79me1 |
| ac4K12acK79me1 | H4Nα-acK16acK79me1 | H4Nα-acK5acK8acK79me1 | H4Nα-acK5b/8acK12ac K79me1 | H4Nα-acK12acK16ac K79me1 | |
| H4Nα-acK5b/8acK16ac K79me1 | H4Nα-acK5acK8ac K12acK79me1 | H4Nα-acK5acK8ac K16acK79me1 | H4Nα-acK5acK12ac K16acK79me1 | H4Nα-acK8acK12acK16acK79me1 | |
| H4Nα-acK5acK8ac K12acK16acK79me1 | H4Nα-acK5acK12acK16acK20acK79me1 | H4Nα-acK5acK8acK12acK16acK20acK79me1 |
a
Proteoforms differing in methionine or cysteine oxidation were not considered. α-amino modification is denoted by “Nα-”, while the other histone PTMs follow standardized nomenclature.36 The names of each protein can be cross-referenced to their gene(s) using Table S1. H3 and H4 refer to the H3.1 and H4.1 sequences, respectively.
b
Indicates ambiguous isomer assignment.
c
Evidence of monoubiquitylation (see Figure S6).