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. 2022 Jun 13;13:889404. doi: 10.3389/fphar.2022.889404

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Copyright © 2022 Gao, Liu, Yao, Zhang, Yang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PNS administration stimulated post-ischemic hippocampal neurogenesis in ME rats. Representative images of ipsilateral hemisphere sctions under a fluorescence microscope at a magnification of ×200 (scale bar =100 μm) were shown as (A) BrdU (red, a marker of proliferating cells) and Nestin (green, a marker of NSC/NPC); (B) BrdU (red) and DCX (green, a marker of migrating neuroblasts); (C) NeuroD1 (green, a marker of differentiation factor). (D) The numbers of double-positive of BrdU/Nestin, BrdU/DCX, and single-positive NeuroD1 were analyzed and data were presented as means ± standard deviation (n = 3 animals per group). *p < 0.05, **p < 0.01 versus Sham group; ^# p < 0.05, ^## p < 0.01 versus ME group.