TABLE 3.
Indicators of 3,4-PCD in the six Roseobacter isolates
| Isolate | Isolation substrate | Growth on POB | 3,4-PCD activitya | Hybridization with pcaH probe | pcaGH retrieved | pcaGH expression in E. coli | pcaH fragment amplifiedb |
|---|---|---|---|---|---|---|---|
| S. stellata E-37 | Indulin | + | + | +c | + | + | + |
| Y3F | Indulin | + | − | +d | + | + | − |
| GAI-16 | Yeast extract | + | + | +c | NAe | NA | − |
| Sulfitobacter sp. strain EE-36 | Indulin | + | − | − | NA | NA | + |
| S25com04 | POB | + | + | +d | NA | NA | − |
| IC4 | Indulin | + | + | − | NA | NA | + |
a
Enzyme activity was detected spectrophotometrically in cell extracts of POB-grown cultures.
b
A 159-bp PCR fragment was amplified using the P34OID primer set.
c
Positive Southern hybridization signal with the pcaHF1 probe was obtained.
d
Positive Southern hybridization signal with the pcaHF1ND probe was obtained.
e
NA, not attempted.