TABLE 4.
β-Ketoadipate pathway genes identified in S. stellata E-37 and Y3F
| Gene designation | Putative function of gene product | Size of gene (bp) | % G+C | Size of deduced gene product
|
Most similar gene, bacterium (% amino acid similarity/identity) GenBank/SwissProt accession no. | |
|---|---|---|---|---|---|---|
| No. of residues | kDa | |||||
| S. stellata E-37 | ||||||
| pobA | p-Hydroxybenzoate hydroxylase | 1,179 | 63.6 | 393 | 44.1 | pobA, P. fluorescens (70/61) (640342) |
| pcaC | γ-Carboxymuconolactone decarboxylase | 393 | 64.9 | 131 | 14.4 | pcaL, S. coelicolor (55/44) (AL079355) |
| pcaG | α subunit of 3,4-PCD | 603 | 65.1 | 201 | 21.9 | pcaG, Acinetobacter sp. strain ADP1 (61/52) (M33798) |
| pcaH | β subunit of 3,4-PCD | 723 | 66.7 | 241 | 26.7 | pcaH, Pseudomonas sp. strain HR199 (70/61) (Y18527) |
| pcaQ | LysR-type transcriptional regulator | 957 | 65.7 | 319 | 34.4 | pcaQ, A. tumefaciens A348 (49/39) (P526681) |
| Y3F | ||||||
| pcaC | γ-Carboxymuconolactone decarboxylase | 396 | 67.6 | 132 | 14.3 | pcaL, S. coelicolor (50/43) (AL079355) |
| pcaG | α subunit of 3,4-PCD | 621 | 64.2 | 207 | 22.9 | pcaG, Acinetobacter sp. strain ADP1 (67/59) (M33798) |
| pcaH | β subunit of 3,4-PCD | 729 | 63.9 | 243 | 26.5 | pcaH, P. putida NCIMB 9869 (69/63) (U96339) |
| pcaQ | LysR-type transcriptional regulator | 927 | 69.1 | 309 | 32.9 | pcaQ, A. tumefaciens A348 (50/43) (P526681) |