TABLE 2.
Recombinant | Plant | Days after planting | % of recombinants | % of active TOM in recombinants | Total no. of organisms (CFU/cm of root or CFU/ml of PPB)a |
---|---|---|---|---|---|
Pb3-1 | Barley | 0 | 58 | 100 | 2.4 × 1010 |
7 | 56 ± 40b | 86 ± 17 | 8.0 × 105 ± 6.5 × 105 | ||
14 | 49 ± 10 | 74 ± 18 | 8.5 × 105 ± 7.3 × 105 | ||
21 | 57 ± 7 | 100 ± 0 | 1.7 × 105 ± 1.7 × 105 | ||
28 | 68 ± 27 | 100 ± 0 | 1.8 × 105 ± 2.5 × 105 | ||
Wheat | 0 | 55 | 100 | 10.4 × 1010 | |
7 | 90 ± 8 | 100 ± 0 | 6.2 × 105 ± 9.4 × 105 | ||
14 | 71 ± 27 | 99 ± 2 | 23 × 105 ± 10 × 105 | ||
21 | 57 ± 23 | 96 ± 6 | 2.2 × 105 ± 1 × 105 | ||
28 | 48 ± 9 | 93 ± 12 | 1.3 × 105 ± 1.5 × 105 | ||
Pb5-1 | Barley | 0 | 47 | 100 | 6.8 × 1010 |
7 | 75 ± 5 | 100 ± 0 | 14 × 105 ± 9 × 105 | ||
14 | 47 ± 24 | 96 ± 4 | 30 × 105 ± 12 × 105 | ||
Wheat | 0 | 47 | 100 | 1.7 × 1010 | |
7 | 46 ± 17 | 100 ± 0 | 25 × 105 ± 19 × 105 | ||
15 | 73 ± 36 | 100 ± 0 | 10 × 105 ± 5 × 105 | ||
21 | 80 ± 21 | 100 ± 0 | 7.2 × 105 ± 6.8 × 105 | ||
28 | 60 ± 10 | 100 ± 0 | 0.98 × 105 ± 0.59 × 105 | ||
Rhizobium sp. strain 35645A | Barley | 0 | 30 | 100 | 29.7 × 1010 |
7 | 54 ± 18 | 39 ± 10 | 8.2 × 105 ± 7 × 105 | ||
14 | 80 ± 10 | 100 ± 0 | 31 × 105 ± 11 × 105 | ||
28 | 51 ± 31 | 91 ± 10 | 3.5 × 105 ± 3.7 × 105 | ||
Wheat | 0 | 63 | 100 | 9.0 × 1010 | |
7 | 24 ± 2 | 96 ± 7 | 53 × 105 ± 26 × 105 | ||
15 | 53 ± 28 | 93 ± 12 | 33 × 105 ± 38 × 105 | ||
21 | 60 ± 24 | 100 ± 0 | 12 × 105 ± 2.7 × 105 | ||
28 | 43 ± 20 | 82 ± 10 | 5.9 × 105 ± 5.4 × 105 | ||
2-79TOMc | Barley | 0 | 28 | 100 | 2.7 × 1010 |
10 | 33 | 33 | 100 × 105 | ||
25 | 31 | 80 | 4 × 105 | ||
Wheat | 0 | 28 | 100 | 2.7 × 1010 | |
10 | 22 | 33 | 50 × 105 | ||
17 | 12 | 63 | 2.2 × 105 | ||
25 | 16 | 33 | 9.5 × 105 | ||
S-16c | Barley | 0 | 55 | 100 | 1.9 × 109 |
10 | 22 | 67 | 50 × 105 | ||
17 | 5 | 23 | 77 × 105 | ||
25 | 16 | 57 | 22 × 105 | ||
Wheat | 0 | 55 | 100 | 1.9 × 109 | |
10 | 20 | 50 | 400 × 105 | ||
17 | 9 | 75 | 17 × 105 | ||
25 | 5 | 67 | 6 × 105 | ||
H-25c | Barley | 0 | 93 | 100 | 5.7 × 1010 |
10 | 75 | 75 | 100 × 105 | ||
17 | 93 | 27 | 52 × 105 | ||
25 | 93 | 86 | 23 × 105 | ||
Wheat | 0 | 93 | 100 | 5.7 × 1010 | |
10 | 35 | 75 | 140 × 105 | ||
17 | 33 | 100 | 1.5 × 105 | ||
25 | 31 | 25 | 6.5 × 105 |
The zero-time data are expressed in CFU per milliliter of PPB; all other data are expressed in CFU per centimeter of root.
Mean ± standard deviation.
Only one 2-cm root sample was analyzed for each plant for these strains on each sampling day.