Immunofluorescent quantification of extra-cellular matrix (A,B) and collagen (C,D) on skeletal muscle cross sections Pre and Post bed rest with NMES+PRO or Control treatment in healthy older adults. Percent change from Pre is shown in panels B and D. ECM was quantitated using wheat germ agglutinin (WGA) and total collagen with sirius red staining. ECM, extra-cellular matrix; NMES, Neuromuscular electrical stimulation; PRO, Protein. Data are Mean ± SEM. # p<0.05 vs Control. % p<0.065 vs Pre.