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CSLM stereo images showing attachment of E. coli O157:H7 on intact apple surface. (A) Cleft (16-μm depth) in the waxy cuticle (open arrow); most cells are attached within the cleft (closed arrow). (B) Clusters of cells (arrow) on intact cuticle 34 μm in height. Cells were inoculated under a negative temperature differential. Bar, 10 μm.
