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. 2022 May 5;11:e76189. doi: 10.7554/eLife.76189

Figure 6. V410A has intrinsically faster microtubule polymerization rates in vitro.

Figure 6.

(A) Graphic depicting aggregates and non-seeded nucleation when tubulin concentrations are too high, no nucleation when concentrations are too low, and dynamic microtubules when concentrations are at an appropriate level. (B) Example kymograph of dynamic microtubule from purified budding yeast tubulin measured using interference reflection microscopy. X and Y scale bars = 5 min and 1 µm, respectively. (C) Microtubule polymerization rates at 0.5, 0.7, and 0.9 µM of tubulin. Each data point represents the mean of three independent experiments; for wild-type (WT) n=15 microtubules at 0.5 µM, n=23 at 0.7 µM, n=18 at 0.9 µM; for V410I n=12 microtubules at 0.5 µM, n=14 at 0.7 µM, n=12 at 0.9 µM; for V410A n=16 microtubules at 0.5 µM, n=20 at 0.7 µM, n=10 at 0.9 µM. Bars are mean ± 95% confidence interval. (D) Microtubule depolymerization rates.

Figure 6—source data 1. In vitro microtubule dynamics.
elife-76189-fig6-data1.xlsx (169.5KB, xlsx)
Figure 6—source data 2. Microtubule dynamics in vitro.
Internal reflection microscopy imaging of purified yeast tubulin assembling from GMPCPP-stabilized seeds made of porcine tubulin.