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. 2022 May 5;11:e76189. doi: 10.7554/eLife.76189

Appendix 1—table 1. Plasmids.

Plasmid ID Plasmid name Source
pJM0157 pGEX6p1-GST-STU2 1–590 Widlund et al., 2012
pJM0295 pCIG2 Hand et al., 2005
pJM0288 pRS426-GAL-Tub1-internal 6X-His Johnson et al., 2011
pJM0289 pRS424-GAL-Tub2 (untagged) Johnson et al., 2011
pJM0430 pCIG2-Tuba1a(WT)-IRES-GFP-MACF43 Aiken et al., 2019
pJM0459 Stu2-3GFP This study
pJM0469 TUB1-pRS306 integrating plasmid Aiken et al., 2020
pJM0477 pCIG2-Tuba1a(V409A)-IRES-GFP-MACF43 This study
pJM0507 Tub1-V410I-pRS306 integrating plasmid This study
pJM0510 pCIG2-Tuba1a(V409I)-IRES-GFP-MACF43 This study
pJM0560 Tub1-V410A-pRS306 integrating plasmid This study
pJM0546 pCIG2-TUBA1A-6X-His Buscaglia et al., 2020a
pJM0558 pRS426-GAL-Tub1(V410A)-internal 6X-His This study
pJM0559 pRS426-GAL-Tub1(V410I)-internal 6X-His This study
pJM0612 pCIG2-TUBA1A(V409I)-int-6X-His This study
pJM0642 pCIG2-TUBA1A(V409A)-int-6X-His This study