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. 2022 May 5;11:e76189. doi: 10.7554/eLife.76189

Appendix 1—table 3. Oligo sequences (5’ to 3’).

# Name Application Sequence Species Direction
674 TUBA1A-V409A F QC TUBA1A-V409A F Quikchange ctttgttcactggtacgctggggaggggatg Human Forward
675 TUBA1A-V409A R QC TUBA1A-V409A R Quikchange catcccctccccagcgtaccagtgaacaaag Human Reverse
869 MACF43 + NotI R PCR up MACF43 and clone into pCIG2 tatc GCGGCCGC TCATCTCTTTGAGGACTTGTCC Mammalian Reverse
870 BsrGI + MACF43 F PCR up MACF43 and clone into pCIG2 GAGC TGTACA AGTCCGGCCGGACTCAG Mammalian Forward
986 TUBA1A-V409I F QC TUBA1A-V409I F Quikchange cctttgttcactggtacattggggaggggatgg Human Forward
987 TUBA1A-V409I R QC TUBA1A-V409I R Quikchange ccatcccctccccaatgtaccagtgaacaaagg Human Reverse
1042 TUBA1A Gibson PstI insert F Amplify TUBA1A from pINCY into pCIG2 ctcaagcttcgaattctgcaATGcgtgagtgcatctccatcc Mouse Forward
1043 TUBA1A Gibson PstI insert R Amplify TUBA1A from pINCY into pCIG2 cggtaccgtcgactgcaTTAgtattcctctccttcttcctcaccc Mouse Reverse
1075 TUBA1A-T349E F QC TUBA1A-T349E F Quikchange gtttgtggattggtgccccgagggcttcaaggttggcatca Human Forward
1076 TUBA1A-T349E R QC TUBA1A-T349E R Quikchange tgatgccaaccttgaagccctcggggcaccaatccacaaac Human Reverse
867 pCIG2-TUBA1A stop +53 R TUBA1A sequencing GGCTTCGGCCAGTAACGTTAG Mammalian Reverse
868 pCIG2-TUBA1A start – 52 F TUBA1A sequencing CCTACAGCTCCTGGGCAACG Mammalian Forward
545 tub1-V410A F tub1-V410A F Quikchange CGTCCACTGGTATGCCGGTGAAGGTATG Yeast Forward
546 tub1-V410A R tub1-V410A R Quikchange CATACCTTCACCGGCATACCAGTGGACG Yeast Reverse
1018 tub1-V410I F tub1-V410I F Quikchange cgtgctttcgtccactggtatatcggtgaaggt Yeast Forward
1019 tub1-V410I R tub1-V410I R Quikchange accttcaccgatataccagtggacgaaagcacg Yeast Reverse
1280 tub3-V410A F tub3-V410A F to introduce mutation at TUB3 locus TGATGTATGC CAAACGTGCT TTCGTCCATT GGTAT GcC GGTGAAGGTATG GAAGAAGGTG Yeast Forward
1281 tub3-V410I F tub3-V410I F to introduce mutation at TUB3 locus TGATGTATGC CAAACGTGCT TTCGTCCATT GGTAT aTC GG TGAAGGTATG GAAGAAGGTG Yeast Forward
5551 TUB3 + 154 R To introduce V410 mutations at TUB3 locus GCTATTGTTCTAACTGTACACCTAAAGGTAAGTG gca tag gcc act agt gga tc Yeast Reverse