Table 8.
Example Anesthetic Protocol for Mouse fMRI Imaging300
| Species: mouse |
| Stock or strain: C57BL/6 |
| Age: 12 wk |
| Sex: male |
| Weight: 30 g |
| Body Condition Score: 3 |
| Procedure needing anesthesia: surgical anesthesia for fMRI |
| Acclimation time: 48 h |
| Fasting time: none |
| Equipment needed: anesthetic machine, circulating water blanket, fiberoptic cable (for body temperature), foam pillow (for respiration), eye lubricant, warmed fluid |
| Pre-medication: none |
| Induction: isoflurane by induction box |
| Maintenance: etomidate CRI (0.75–1.5 mg/kg/min) after etomidate initial bolus (4 mg/kg/min over first 3 min) |
| Recovery: in home cage on circulating water blanket |