FIG. 4.

Compatible solute uptake in EGD (circles) or in the opuCA::pAULA mutant derivative (squares). Cultures were grown in DMP prior to the assay. The assay was performed with potassium phosphate buffer with (solid symbols) or without (open symbols) 0.5 M added NaCl. The assay was performed in the presence of the protein synthesis inhibitor chloramphenicol (50 μg ml⁻¹) and with 0.4% (wt/vol) added glucose. l-[³H]carnitine hydrochloride (a) or l-[¹⁴C]betaine (b) was added to a final concentration of 20 μM.