Hindlimb unloading (HU) does not change the expression of glutamic acid decarboxylase 67 (GAD67) and vesicular GABA transporter (VGAT). A: immunofluorescence image of GAD67 (green) and VGAT (orange) in the nucleus tractus solitarii (nTS) region of a representative rat from control group. B and C: mean data showing comparable values of fluorescent intensity of GAD67 (control: N = 6, HU: N = 6) and VGAT (control: N = 5, HU: N = 5) in HU and control. Immunoblot quantification of GAD67/total protein ratio (D, control: N = 8, HU: N = 7) and VGAT/total protein ratio (E, control: N = 8, HU: N = 8) confirmed that expression of these proteins was not altered by HU.