Table 1.

A selection of mouse hindlimb muscles and a range of parameters used in electroporation studies for gene delivery

	Mouse Skeletal Muscle
	Tibialis Anterior	Gastrocnemius	Plantaris	Quadriceps
Hyaluronidase (Optional)	0.4 U/µL (30 µL)	0.4 U/µL (40 µL × 2) for each lateral and medial head
Surgery	Optional—small incision or blind injection	No	Yes	Yes
Plasmid volume	5–20 µg (30 µL)	5–50 µg (40 µL × 2) for lateral and medial head	12 µL (2 × 6 µL)	50 µg (50 µL)
Injection number	1 to 2	1 to 2	2	1
Syringe needle	28-G	28-G	27-G	28-G
Pulses	5–10	8–10	8	8
Voltage, V/cm	50–175	175–200	180–160	150–200
Pulse width, ms	10–40	20–100	20	20–50
Electrode type	7-mm plate or needle	10-mm plate	Needle	10-mm needle
Reference	(51, 54, 57, 58, 66–68, 84, 85)	(84, 86)	(87)	(88–93)