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. 2022 May 27;323(1):H146–H164. doi: 10.1152/ajpheart.00105.2022

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Figure 6. — Tri-omics analysis of significant changes in the cardiac transcriptome, metabolome and proteome changes in during pregnancy. ANOVA analysis was performed to identify significantly expressed cardiac transcripts, metabolites and protein abundances [P <0.05; false discovery rate (FDR) 0.05] across all time points [nonpregnant, diestrus (NP), midpregnant (MP; day 8 of pregnancy), late pregnant (LP; day 16 of pregnancy), and 1 wk postbirth with lactation (PB)]. Official gene names and Human Metabolome Database (HMDB) and Uniprot IDs of those significantly expressed targets were added to the tri-omics analysis software OmicsNet. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was performed to analyze significantly impacted pathways using KEGG gene + metabolite (G+M; A), KEGG gene (B), and KEGG metabolite (C).