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Identification of PDLSCs. (A) The morphology of PDLSCs was observed under a microscope. (B) Colonies formed by PDLSCs after 14 days of culture were stained with Giemsa stain. (C) Alizarin Red staining of calcium deposits formed by PDLSCs after incubation in osteogenic induction medium for 3 weeks. (D) Flow cytometric analysis was applied for examining the mesenchymal stem cell (MSC) phenotype of PDLSCs.
