Figure 5.

Characterization of estrogenic zones 11–13 directly out of the pYES bioautogram

(A) Chromatogram at FLD 366 nm on HPTLC plate silica gel 60 with ethyl acetate – methanol – water 15:3:1 (V/V/V) of the extracts of the three distinct substrates, (B) pYES bioautogram, and (C) elution head imprint, which verifies the proper positioning of the elution head on the zones. (D) Scheme of the zone characterization by NP-HPTLC-pYES bioassay-RP-HPLC-DAD-HESI-MS showing recorded PDA, MS-TIC chromatograms and extracted mass spectra (same colour) with assigned mass signals.