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. 2022 Jun 27;18(7):774–781. doi: 10.1038/s41589-022-01054-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a, Complementation of the LSS-deficient yeast strain (Gil77) with echinoderm OSCs. Yeast transformants spotted undiluted (−), 10- and 100-folds dilution and growth recorded 7 days after spotting. GC-MS total ion chromatograms (TICs) of yeast extracts expressing b, sea star PmLSS and sea urchin SpLSS, c, sea cucumber LDS, d, sea cucumber PS. Superscripts ‘a’ and ‘b’ indicate different accessions of A. japonicus. Peaks marked with asterisks in c and d indicate background modifications of OSC products in yeast. e, GC-MS TICs of yeast extract expressing AjLDS^b treated with different concentrations of ketoconazole (CYP51 inhibitor) in the medium (1-50 µg/ml). f-h, GC mass spectra of lanosterol (2), lanostadienol (4) and parkeol (3). pYES2, yeast containing the empty vector (ev). Standards, 2, lanosterol; 4, lanostadienol; 3, parkeol. Ergo = ergosterol, glu = glucose and gal = galactose.