Fig. 7. Low mitochondrial content in pheochromocytoma cells causes impaired differentiation.

a, Fluorescence images of PC12 cells treated with 50 ng ml⁻¹ NGF at the indicated time points. Cells were stained by MitoTracker Red to visualize mitochondria and endogenous Tuj1 (neuron-specific class III beta-tubulin) was stained in green. b, Corresponding immunoblot analysis. n = 3 biological independent experiments. c, Fluorescence images of stable polyclonal PC12 cells expressing the indicated human VHL (huVHL) species selected with G418 (0.5 mg ml⁻¹) for 2 weeks. PC12 clones were transduced for 48 h with lentivirus encoding shRNA targeting endogenous rat VHL (sh-ratVHL) or scramble control (shSCR) and subsequently treated with NGF for 6 d. Cells were stained by MitoTracker Red to visualize mitochondria and endogenous Tuj1 was stained in green. d, Fluorescence images of polyclonal PC12 cells transduced for 48 h with lentivirus encoding shRNA targeting endogenous rat TFAM (sh-TFAM) or scramble control (shSCR) and subsequently treated with NGF for 6 d. Cells were stained by MitoTracker Red to visualize mitochondria and endogenous Tuj1 in green. e, Corresponding immunoblot analysis. n = 3 biological independent experiments. In a, c and d, similar results were observed more than three times.

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