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. 2022 Jun 27;4(6):693–710. doi: 10.1038/s42255-022-00582-0

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© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, ¹³C-labelled metabolite incorporation in cCAF cultured in DMEM or physiol. DMEM (5 mM glucose, 0.65 mM glutamine, 100 µM pyruvate and 100 µM acetate). b, Acetyl-CoA production/export from mitochondria. c, Unlabelled (black) and ¹³C-labelled (coloured) acetyl-CoA in ¹³C-glucose labelled cCAFs with 50 μM BMS303141/DMSO control and 1 mM acetate/PBS control. d,e, Representative western blot (d) and quantification (e) showing H3K27ac in CAFs with 50 μM BMS303141/DMSO control and 1 mM acetate/PBS control. n = 4–6 biological replicates. f, Representative western blot of PYCR1 in cCAFs with 50 μM BMS303141/DMSO control and 1 mM acetate/PBS control (1 of 3). g, mRNA quantification in cCAFs with 50 μM BMS303141/DMSO control and 1 mM acetate/PBS control. n = 3 biological replicates. h, Collagen quantification with 1 mM acetate/PBS control. n = 3 biological replicates. i,j, Representative images (j) and quantification (i) of collagen in 2D cCAF/cancer cell cocultures with 50 μM BMS303141/DMSO control and 1 mM acetate/PBS control. n = 3 biological replicates. k,l, Representative images (k) and quantification (l) of collagen in 3D cCAF/cancer cell cocultures with BMS303141/DMSO control and 1 mM acetate/PBS control. n = 3 biological replicates. m,n, Representative western blot (m) and quantification (n) of COL6A1 in ECM from cCAFs with 50 μM BMS303141/DMSO control and 1 mM acetate/PBS control. n = 3 biological replicates. o,p, Proliferation of cancer cells (o) and CAFs (p) in cocultures treated with 50 μM BMS303141/DMSO control. n = 3 biological replicates. q, Proliferation of cancer cells seeded on ECM from cCAFs treated with 50 μM BMS303141/DMSO control and 1 mM acetate/PBS control. n = 3 biological replicates. Error bars indicate mean ± s.e.m. P values were calculated with two-tailed, unpaired t-test with Welch’s correction (a), two-tailed Mann–Whitney test (o and p), one-way ANOVA with Dunnett’s multiple comparison test (c, g, i, l and n) or Kruskal–Wallis with Dunn’s multiple comparison test (e and q). Scale bar, 200 µm. See Extended Data Fig. 9 for Ponceau S staining of blots used for COL6A1 in ECM, used as loading control.

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