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. 2022 May 12;17(3):1378–1386. doi: 10.1016/j.jds.2022.04.017

Figure 2.

Figure 2

IGF-1 and TGF-β mRNA expression and specific marker gene expression profile in immortalized TMJ fibrocartilage cells. (A) Analyses of gene expression levels for IGF-1 and TGF-β were performed by qRT-PCR. IGF-1 and TGF-β mRNA levels were compared between early passage and late passage cells with immortalized TMJ fibrocartilage cells. (B) The effect of growth factors on immortalized TMJ fibrocartilage cells. The immortalized TMJ fibrocartilage cells on late passage were cultured in the absence or presence of 100 ng/ml of BMP-2 or 10 ng/ml of TGF-β in normal medium for 24 h. Relative gene expression analysis of Aggrecan, Col2a1 and Sox9 was performed by quantitative qRT-PCR. Agc, Col2a1 and Sox9 mRNA were detected after treatment with TGF-β and the level of mRNA of Col2a1 and Sox9 were highly increased by TGF-β treatment. BMP-2 increased the level of Agc, Col2a1 and Sox9 mRNA in immortalized TMJ fibrocartilage cells. IGF indicates Insulin-like growth factor 1; TGF, transforming growth factor; BMP2, Bone morphogenetic protein 2; Agc, Aggrecan; Col2a1, type II collagen. All experiments were normalized to GAPDH and statistical significance was assessed by student t-test (∗P < 0.05, ∗∗P < 0.0001).