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. 2022 May 12;17(3):1378–1386. doi: 10.1016/j.jds.2022.04.017

Figure 3.

Figure 3

Expression of cartilage markers and degradation enzymes. (A) The TMJ fibrocartilage cells on late passage were cultured in the absence or presence of 10 ng/ml IL-1β or 1 μM BIO, GSK3β inhibitor for 24 h. IL1β highly decreased the level of expression of Agc but BIO decreased Sox9 mRNA. (B) The mRNA level of Mmp13 and Adamts5, degradation enzymes that are related to the onset of articular cartilage degeneration, was determined using qRT-PCR after 24 h in the absence or presence of 10 ng/ml IL-1β or 1 μM BIO. Mmp13 expression was increased by IL1β or BIO treatment but Adamts5 was not changed after treatment. Mmp13 indicates Matrix Metalloproteinase 13; Adamts5, A disintegrin and metalloproteinase with thrombospondin motifs 5. Experiments were performed more than three times from different passages and normalized to GAPDH and statistical significance was assessed by student t-test (∗P < 0.0001).