Table 1.
Primer sequences for qPCR.
| Primers | Forward (5′→3′) | Reverse (5′→3′) |
|---|---|---|
| Aggrecan | AGGATGGCTTCCACCAGTGC | TGCGTAAAAGACCTCACCCTCC |
| Col2a1 | CCTGGACCCCGTGGCAGAGA | CAGCCATCTGGGCTGCAAAG |
| SOX9 | TAAATTCCCAGTGTGCATCC | GCACCAGGGTCCAGTCATA |
| β-catenin | GACAGAGTTGCTCCACTCCA | TGGCTTGTCCTCAGACATTC |
| Adamts5 | GGCTGTGGTGTGCTGTG | CTGGTCTTTGGCTTTGAAC |
| MMP13 | GCAGCTCCAAAGGCTACAA | CATCATCTGGGAGCATGAAA |
| TGF-β | CTTTGTACAACAGCACCCGC | TAGATTGCGTTGTTGCGGTC |
| IGF-1 | ATGAGCGCACCTCCAATAAAGA | ACGAACTGAAGAGCGTCCAC |
| GAPDH | CCACAGTCCATGCCATCAC | TCCACCACCCTGTTGCTGTA |
Abbreviations: Col2a1, Collagen, type II, alpha 1; SOX9, SRY-Box Transcription Factor 9; Adamts5, The abnormal secretion of disintegrins and matrix metalloproteinases (MMPs) with thrombospondin motifs 5; MMP13, Matrix metalloproteinase 13; TGF-β, Transforming growth factor beta; IGF-1, Insulin-like growth factor 1; GAPDH, Glyceraldehyde-3-Phosphate Dehydrogenase.