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. 2022 May 21;22(8):701–712. doi: 10.1007/s12012-022-09746-6

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — SRSF6 phosphorylation in iPSC cardiomyocytes overexpressing DYRK1A. A Evaluation of the anti-phosphoepitope SR antibody in total protein extracts from AC16 cardiomyocytes treated with calf intestine alkaline phosphatase (CIAP). B Detection of phospho-SRSF6 and SRSF6 in iPSC cardiomyocytes transfected with empty vector (EV), SRSF6-HA expression construct (SRSF6) or co-transfected with DYRK1A and SRSF6-HA (SRSF6 + DYRK1A). β-Tubulin was assayed as loading control. Lower panel: densitometric analysis. Upper right panel: relative DYRK1A mRNA fold expression. (A.U.: arbitrary units). C Detection of phosphorylated SRSF6 in immunoprecipitated samples from iPSC cardiomyocytes. Right panel: densitometric analysis. Horizontal bars show the mean ± SD from determinations performed in quintuplicate. D Dot-blot analysis of phosphorylated SRSF6 in immunoprecipitated samples. Right panel: densitometric analysis. Horizontal bars show the mean ± SD from determinations performed in quintuplicate. ***P < 0.001, *P < 0.05, Student’s t test