Table 2.
Triglyceride, cholesteryl ester, free cholesterol and total cholesterol (which is the sum of cholesteryl ester and free cholesterol) in Hepa1-6 cells, Huh7 cell and HepG2 cells transfected with the corresponding control plasmid (control) or a plasmid to express muChem-156 or huChem-157
| Triglycerides (nmol/mg protein) | Cholesteryl ester (nmol/mg protein) | Free cholesterol (nmol/mg protein) | Total cholesterol (nmol/mg protein) | |
|---|---|---|---|---|
| Hepa1-6 | ||||
| Control | 3.3 (2.0–6.5) | 2.8 (1.8–4.8) | 47.8 (28.4–66.5) | 51.4 (30.2–69.9) |
| muChem-156 | 4.8 (4.5–5.2) | 3.3 (2.8–4.4) | 56.1 (51.7–70.5) | 59.7 (55.0–70.5) |
| Huh7 | ||||
| Control | 91.9 (80.5–103.2) | 27.5 (25.0–28.4) | 73.6 (60.9–84.8) | 101.1 (89.2–109.8) |
| huChem-157 | 86.6 (51.3–175.4) | 25.5 (14.0–47.3) | 69.4 (47.6–130.5) | 94.9 (61.6–177.8) |
| HepG2 | ||||
| Control | 43.5 (39.3–73.7) | 19.6 (9.7–31.2) | 54.0 (22.0–85.3) | 73.6 (31.7–116.5) |
| huChem-157 | 58.4 (31.1–135.2) | 24.0 (10.8–30.6) | 72.4 (35.7–86.0) | 96.4 (46.6–116.6) |
Median values, minimum and maximum are given (n = 4). There was no effect of chemerin overexpression on the levels of these lipids in any cell line