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. 2022 Jun 27;13:3685. doi: 10.1038/s41467-022-31322-3

Fig. 8. De novo L1-ORFeus insertions are rare in base editing.

Fig. 8

a Diagram of the base editors. BE2, BE3, BE4-Gam, and AncBE4max are developed as cytosine base editors (CBEs), while ABE8e is an adenine base editor (ABE). b, c Numbers of L1-ORFeus insertions obtained by amplicon sequencing at the MYC (b) or FANCF (c) base editing site targeted by BE2, BE3, BE4-Gam, AncBE4max, or ABE8e in HEK293T cells expressing L1-ORFeus or L1-RTm. d Numbers of L1-ORFeus insertions obtained by amplicon sequencing at the BCL11A enhancer or HBG1/2 promoter (−198 bp/−175 bp target) base editing site targeted by ABE8e in HEK293T cells expressing L1-ORFeus or L1-RTm. e Detailed view of the distribution of L1-ORFeus insertions at the MYC base editing site obtained by PolyA-seq in HEK293T cells targeted by AncBE4max or ABE8e that express L1-ORFeus or L1-RTm. f Detailed view of the distribution of L1-ORFeus insertions at the BCL11A enhancer base editing site obtained by PolyA-seq in HEK293T cells targeted by ABE8e that express L1-ORFeus or L1-RTm.