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. 2022 Jun 27;12:10877. doi: 10.1038/s41598-022-14860-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Lonp1 localizes in the nuclei of mouse colon tissue and human colon tissue. (A) Representative images of Lonp1 IHC on murine colon tissue, showing Lonp1 nuclear localization. (B) Representative images of Lonp1 IF on murine colon tissue, showing Lonp1 nuclear localization. Nuclei were counterstained with DAPI. (C) Representative Ki-67 IHC staining in intestinal crypt. Cells of the crypt were sectioned into three zones: the lower (0 to + 4), upper (+ 5 to + 15), and non (> + 15)-crypt zones. The histogram represents the percentage of cells with nuclear Lonp1 per crypt zone (n = 10 crypts per group). Data represent the mean ± SD. (D) Representative images of Lonp1 IHC on human colon tissue, showing Lonp1 nuclear localization (arrows). Bar: 200 µm.