TABLE 2.
Wood decay and detection of fungal species in wood blocks after 8 months of colonization
| Species | Isolate | Mean % wt loss of wood ± SDa | PCR amplificationb with primer pair:
|
|
|---|---|---|---|---|
| ITS1-F–ITS4 | ITS1-F–ITS4-B | |||
| Uninoculated control wood | 0.1 ± 0.3 | ++ | −− | |
| Brown-rot basidiomycetes | ||||
| Coniophora puteana | Fp-90099-Sp | 0.1 ± 0.3 | ++ | ++ |
| Postia placenta | Mad-698-R | 65.5 ± 1.2 | ++ | ++ |
| Postia placenta | 65.8 ± 1.4 | ++ | ++ | |
| Gloeophyllum trabeum | 67.3 ± 3.3 | ++ | ++ | |
| Gloeophyllum trabeum | Mad-617-R | 69.6 ± 1.8 | ++ | ++ |
| Gloeophyllum sepiarum | 10-BS2-2 | 68.1 ± 0.9 | ++ | ++ |
| Leucogyrophana pinastri | 68.1 ± 4.8 | ++ | ++ | |
| Serpula lacrimans | Harm-888-R | 67.5 ± 1.9 | ++ | ++ |
| White-rot basidiomycetes | ||||
| Lentinula edodes | 117=1t(d) | 3.0 ± 1.7 | ++ | ++ |
| Trametes versicolor | 35.1 ± 13.6 | ++ | ++ | |
| Trametes versicolor | Fp-101664-Sp | 0.0 ± 0.1 | ++ | ++ |
| Irpex lacteus | KTS 003 | 40.1 ± 12.1 | ++ | ++ |
| Resinicium bicolor | HHB-8850-sp | 10.4 ± 0.9 | ++ | ++ |
| Resinicium bicolor | ATCC 44175 | 3.7 ± 3.6 | ++ | ++ |
| Resinicium bicolor | ATCC 64897 | 12.1 ± 5.7 | ++ | ++ |
| Scytinostroma galactinum | MB-1880-sp | −0.8 ± 0.5 | ++ | ++ |
| Scytinostroma galactinum | ATCC 64896 | 1.1 ± 0.3 | ++ | ++ |
| Scytinostroma galactinum | ATCC 44178 | −0.2 ± 0.7 | ++ | ++ |
| Phanerochaete chrysosporium | 9.9 ± 11.8 | ++ | ++ | |
| Phanerochaete chrysosporium | ATCC 24725 | 14.2 ± 5.7 | ++ | ++ |
| Wood-inhabiting ascomycetes | ||||
| Phialophora mutabilis | ATCC 42792 | 0.4 ± 0.2 | ++ | −− |
| Trichoderma reesei | ATCC 26921 | −0.2 ± 0.4 | ++ | −− |
| Trichoderma viride | ATCC 32630 | −0.1 ± 0.1 | ++ | −− |
| Hormonema dematiodes | 0.4 ± 0.0 | ++ | −− | |
| Pestolotiopsis sp. | 0.6 ± 0.0 | ++ | −− | |
| Xenomeris abietis | 0.5 ± 0.0 | ++ | −− | |
| Aureobasidium pullulans | ATCC 34621 | 0.2 ± 0.0 | ++ | −− |
| Phialocephala fusca | ATCC 62326 | −0.2 ± 0.0 | ++ | −− |
| Ceratocystis pilifera | ATCC 60758 | 2.7 ± 0.0 | ++ | −− |
| Ophiostoma ulmi | ATCC 32439 | 0.1 ± 0.0 | ++ | −− |
a
Mean of two replicate spruce wood blocks.
b
Primer ITS1-F is specific for higher fungi, primer ITS4 is a universal primer, and primer ITS4-B is specific for basidiomycetes. Each plus or minus sign represents the amplification results for an individual wood block.